Vascular leakage in different stages in MMTV-PyMT mice
To visualize differences in vascular leakage between different tumor stages, an MMTV-PyMT;ACTB-ECFP mouse was intravenously injected with 10 kD dextran (red) and 2 MD dextran (green). Shown are three different fields of view imaged in the same mouse, corresponding to hyperplasia (left), early carcinoma (middle), and late carcinoma (right). The early carcinoma showed the greatest degree of vascular leakage, with the hyperplasia showing limited leakage and the late carcinoma showing leakage primarily at the tumor-stroma border. Time after injection with dextrans is indicated. Scale bars: 100 microm.
From Nakasone et al. (2012) Cancer Cell, 21(4):488-503.